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alexa fluor  (Vector Laboratories)


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    Vector Laboratories alexa fluor
    Alexa Fluor, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/L-1390-2/Unconjugated+Aleuria+Aurantia+Lectin+(AAL)/pm41577690-281-4-9
    Average 94 stars, based on 230 article reviews
    alexa fluor - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Immunopeptidomics:

    Article Title: Studying Macromolecular Composition in Cell-Cell Interfaces Using 3D Membrane Reconstitution Systems.
    Article Snippet: We used the following recombinant proteins obtained from Sino Biological: human CD2 (His-Tag,10982-H08H), CD58/ human LFA-3 (His-Tag, 12409-H08H), PD1/human PDCD1 (His-Tag,10377-H08H), PD-L1/human B7-H1/ CD274 (His-Tag, 10084-H08H), CD80/human B7-1 (His-Tag, 10698-H08H), human CTLA-4 (His-Tag,11159-H08H), NCR3/human NKp30 (His-Tag, 10480-H08H), human B7-H6 (His-Tag, 16140-H08H), human SLAMF6/human Ly108 (His-Tag, 11945-H08H), human CD84 (His-Tag, 10100-H08H), human SIRP αV2 (His-Tag, 30014-H08H), human CD47 (His-Tag, 12283-H08H), CD45 (ECD, His-Tag, 14197- H08H). .. We also used the proteins: CD43/ human leukosialin (His-Tag, CD3-H52H9, ACROBiosystems), MUC1-Alexa Fluor 488 (kindly provided by Carolyn Shurer), MHC class I H-2Db presenting the LCMV-derived gp33 peptide (KAVYNFATM) [ 37, 38 ], MHC class I (HLA-A*02:01) presenting the tumor-associated antigen NY-ESO1-9 V peptide (SLLMWITQV; from now on 9 V) [ 39 ], MHC class I (HLA-A*02:01) presenting the NY-ESO1-3P9V peptide variant (SLPMWITQV; from now on 3P9V), P14 TCR [ 38 ], 1G4 TCR [ 39 ], Wheat Germ Agglutinin (WGA) Alexa Fluor 647 Conjugate (ThermoFisherScientific), Aleuria Aurantia Lectin (AAL) (L-1390-2, Vector Labs), Sambucus Nigra-I-Agglutinin (SNA) (L-1300-5, Vector Labs), Maackia Amurensis-II Lectin (MAL-II) (L-1260-2, Vector Labs). .. We used Alexa Fluor 488 or 647 NHS Ester (Succinimidyl Ester) (ThermoFisher Scientific) for protein labeling.

    Article Title: Studying macromolecular composition in cell-cell interfaces using 3D membrane reconstitution systems
    Article Snippet: We used the following recombinant proteins obtained from Sino Biological: human CD2 (His-Tag,10982-H08H), CD58/ human LFA-3 (His-Tag, 12409-H08H), PD1/human PDCD1 (His-Tag,10377-H08H), PD-L1/human B7-H1/ CD274 (His-Tag, 10084-H08H), CD80/human B7-1 (His-Tag, 10698-H08H), human CTLA-4 (His-Tag,11159-H08H), NCR3/human NKp30 (His-Tag, 10480-H08H), human B7-H6 (His-Tag, 16140-H08H), human SLAMF6/human Ly108 (His-Tag, 11945-H08H), human CD84 (His-Tag, 10100-H08H), human SIRPαV2 (His-Tag, 30014-H08H), human CD47 (His-Tag, 12283-H08H), CD45 (ECD, His-Tag, 14197-H08H). .. We also used the proteins: CD43/ human leukosialin (His-Tag, CD3-H52H9, ACROBiosystems), MUC1-Alexa FluorTM 488 (kindly provided by Carolyn Shurer), MHC class I H-2D b presenting the LCMV-derived gp33 peptide (KAVYNFATM) ( ; ), MHC class I (HLA-A*02:01) presenting the tumour-associated antigen NY-ESO1-9V peptide (SLLMWITQV; from now on 9V) , MHC class I (HLA-A*02:01) presenting the NY-ESO1-3P9V peptide variant (SLPMWITQV; from now on 3P9V), P14 TCR , 1G4 TCR , Wheat Germ Agglutinin (WGA) Alexa FluorTM 647 Conjugate (ThermoFisherScientific), Aleuria Aurantia Lectin (AAL) (L-1390-2, Vector Labs), Sambucus Nigra-I-Agglutinin (SNA) (L-1300-5, Vector Labs), Maackia Amurensis-II Lectin (MAL-II) (L-1260-2, Vector Labs). .. We used Alexa FluorTM 488 or 647 NHS Ester (Succinimidyl Ester) (ThermoFisher Scientific) for protein labelling.

    Variant Assay:

    Article Title: Studying Macromolecular Composition in Cell-Cell Interfaces Using 3D Membrane Reconstitution Systems.
    Article Snippet: We used the following recombinant proteins obtained from Sino Biological: human CD2 (His-Tag,10982-H08H), CD58/ human LFA-3 (His-Tag, 12409-H08H), PD1/human PDCD1 (His-Tag,10377-H08H), PD-L1/human B7-H1/ CD274 (His-Tag, 10084-H08H), CD80/human B7-1 (His-Tag, 10698-H08H), human CTLA-4 (His-Tag,11159-H08H), NCR3/human NKp30 (His-Tag, 10480-H08H), human B7-H6 (His-Tag, 16140-H08H), human SLAMF6/human Ly108 (His-Tag, 11945-H08H), human CD84 (His-Tag, 10100-H08H), human SIRP αV2 (His-Tag, 30014-H08H), human CD47 (His-Tag, 12283-H08H), CD45 (ECD, His-Tag, 14197- H08H). .. We also used the proteins: CD43/ human leukosialin (His-Tag, CD3-H52H9, ACROBiosystems), MUC1-Alexa Fluor 488 (kindly provided by Carolyn Shurer), MHC class I H-2Db presenting the LCMV-derived gp33 peptide (KAVYNFATM) [ 37, 38 ], MHC class I (HLA-A*02:01) presenting the tumor-associated antigen NY-ESO1-9 V peptide (SLLMWITQV; from now on 9 V) [ 39 ], MHC class I (HLA-A*02:01) presenting the NY-ESO1-3P9V peptide variant (SLPMWITQV; from now on 3P9V), P14 TCR [ 38 ], 1G4 TCR [ 39 ], Wheat Germ Agglutinin (WGA) Alexa Fluor 647 Conjugate (ThermoFisherScientific), Aleuria Aurantia Lectin (AAL) (L-1390-2, Vector Labs), Sambucus Nigra-I-Agglutinin (SNA) (L-1300-5, Vector Labs), Maackia Amurensis-II Lectin (MAL-II) (L-1260-2, Vector Labs). .. We used Alexa Fluor 488 or 647 NHS Ester (Succinimidyl Ester) (ThermoFisher Scientific) for protein labeling.

    Article Title: Studying macromolecular composition in cell-cell interfaces using 3D membrane reconstitution systems
    Article Snippet: We used the following recombinant proteins obtained from Sino Biological: human CD2 (His-Tag,10982-H08H), CD58/ human LFA-3 (His-Tag, 12409-H08H), PD1/human PDCD1 (His-Tag,10377-H08H), PD-L1/human B7-H1/ CD274 (His-Tag, 10084-H08H), CD80/human B7-1 (His-Tag, 10698-H08H), human CTLA-4 (His-Tag,11159-H08H), NCR3/human NKp30 (His-Tag, 10480-H08H), human B7-H6 (His-Tag, 16140-H08H), human SLAMF6/human Ly108 (His-Tag, 11945-H08H), human CD84 (His-Tag, 10100-H08H), human SIRPαV2 (His-Tag, 30014-H08H), human CD47 (His-Tag, 12283-H08H), CD45 (ECD, His-Tag, 14197-H08H). .. We also used the proteins: CD43/ human leukosialin (His-Tag, CD3-H52H9, ACROBiosystems), MUC1-Alexa FluorTM 488 (kindly provided by Carolyn Shurer), MHC class I H-2D b presenting the LCMV-derived gp33 peptide (KAVYNFATM) ( ; ), MHC class I (HLA-A*02:01) presenting the tumour-associated antigen NY-ESO1-9V peptide (SLLMWITQV; from now on 9V) , MHC class I (HLA-A*02:01) presenting the NY-ESO1-3P9V peptide variant (SLPMWITQV; from now on 3P9V), P14 TCR , 1G4 TCR , Wheat Germ Agglutinin (WGA) Alexa FluorTM 647 Conjugate (ThermoFisherScientific), Aleuria Aurantia Lectin (AAL) (L-1390-2, Vector Labs), Sambucus Nigra-I-Agglutinin (SNA) (L-1300-5, Vector Labs), Maackia Amurensis-II Lectin (MAL-II) (L-1260-2, Vector Labs). .. We used Alexa FluorTM 488 or 647 NHS Ester (Succinimidyl Ester) (ThermoFisher Scientific) for protein labelling.

    Whole Genome Amplification:

    Article Title: Studying Macromolecular Composition in Cell-Cell Interfaces Using 3D Membrane Reconstitution Systems.
    Article Snippet: We used the following recombinant proteins obtained from Sino Biological: human CD2 (His-Tag,10982-H08H), CD58/ human LFA-3 (His-Tag, 12409-H08H), PD1/human PDCD1 (His-Tag,10377-H08H), PD-L1/human B7-H1/ CD274 (His-Tag, 10084-H08H), CD80/human B7-1 (His-Tag, 10698-H08H), human CTLA-4 (His-Tag,11159-H08H), NCR3/human NKp30 (His-Tag, 10480-H08H), human B7-H6 (His-Tag, 16140-H08H), human SLAMF6/human Ly108 (His-Tag, 11945-H08H), human CD84 (His-Tag, 10100-H08H), human SIRP αV2 (His-Tag, 30014-H08H), human CD47 (His-Tag, 12283-H08H), CD45 (ECD, His-Tag, 14197- H08H). .. We also used the proteins: CD43/ human leukosialin (His-Tag, CD3-H52H9, ACROBiosystems), MUC1-Alexa Fluor 488 (kindly provided by Carolyn Shurer), MHC class I H-2Db presenting the LCMV-derived gp33 peptide (KAVYNFATM) [ 37, 38 ], MHC class I (HLA-A*02:01) presenting the tumor-associated antigen NY-ESO1-9 V peptide (SLLMWITQV; from now on 9 V) [ 39 ], MHC class I (HLA-A*02:01) presenting the NY-ESO1-3P9V peptide variant (SLPMWITQV; from now on 3P9V), P14 TCR [ 38 ], 1G4 TCR [ 39 ], Wheat Germ Agglutinin (WGA) Alexa Fluor 647 Conjugate (ThermoFisherScientific), Aleuria Aurantia Lectin (AAL) (L-1390-2, Vector Labs), Sambucus Nigra-I-Agglutinin (SNA) (L-1300-5, Vector Labs), Maackia Amurensis-II Lectin (MAL-II) (L-1260-2, Vector Labs). .. We used Alexa Fluor 488 or 647 NHS Ester (Succinimidyl Ester) (ThermoFisher Scientific) for protein labeling.

    Article Title: Studying macromolecular composition in cell-cell interfaces using 3D membrane reconstitution systems
    Article Snippet: We used the following recombinant proteins obtained from Sino Biological: human CD2 (His-Tag,10982-H08H), CD58/ human LFA-3 (His-Tag, 12409-H08H), PD1/human PDCD1 (His-Tag,10377-H08H), PD-L1/human B7-H1/ CD274 (His-Tag, 10084-H08H), CD80/human B7-1 (His-Tag, 10698-H08H), human CTLA-4 (His-Tag,11159-H08H), NCR3/human NKp30 (His-Tag, 10480-H08H), human B7-H6 (His-Tag, 16140-H08H), human SLAMF6/human Ly108 (His-Tag, 11945-H08H), human CD84 (His-Tag, 10100-H08H), human SIRPαV2 (His-Tag, 30014-H08H), human CD47 (His-Tag, 12283-H08H), CD45 (ECD, His-Tag, 14197-H08H). .. We also used the proteins: CD43/ human leukosialin (His-Tag, CD3-H52H9, ACROBiosystems), MUC1-Alexa FluorTM 488 (kindly provided by Carolyn Shurer), MHC class I H-2D b presenting the LCMV-derived gp33 peptide (KAVYNFATM) ( ; ), MHC class I (HLA-A*02:01) presenting the tumour-associated antigen NY-ESO1-9V peptide (SLLMWITQV; from now on 9V) , MHC class I (HLA-A*02:01) presenting the NY-ESO1-3P9V peptide variant (SLPMWITQV; from now on 3P9V), P14 TCR , 1G4 TCR , Wheat Germ Agglutinin (WGA) Alexa FluorTM 647 Conjugate (ThermoFisherScientific), Aleuria Aurantia Lectin (AAL) (L-1390-2, Vector Labs), Sambucus Nigra-I-Agglutinin (SNA) (L-1300-5, Vector Labs), Maackia Amurensis-II Lectin (MAL-II) (L-1260-2, Vector Labs). .. We used Alexa FluorTM 488 or 647 NHS Ester (Succinimidyl Ester) (ThermoFisher Scientific) for protein labelling.

    Incubation:

    Article Title: Plant fucosyltransferase FUT11 distorts the sugar acceptor to catalyze via a transient oxocarbenium intermediate mechanism
    Article Snippet: .. Solutions of fluorescently labelled (Alexa Fluor-555) Aleuria aurantia lectin (Vector Lab, L-1390-2, 1.4 μM) and Anti-HRP (Merck, P7899, 2.0 μM) in TBST (50 mM TRIS, 150 mM NaCl, pH = 7.5 + 0.01% Tween 20) were incubated in the dark at room temperature for 1 hour. ..

    Article Title: Plant fucosyltransferase FUT11 distorts the sugar acceptor to catalyze via a transient oxocarbenium intermediate mechanism.
    Article Snippet: .. Solutions of fluorescently labelled (Alexa Fluor-555) Aleuria aurantia lectin (Vector Lab, L-1390-2, 1.4 μM) and Anti-HRP (Merck, P7899, 2.0 μM) in TBST (50 mM TRIS, 150 mM NaCl, pH=7.5 +0.01% Tween 20) were incubated in the dark at room temperature for 1 hour. ..

    other:

    Article Title: Single-cell mapping of the glycocalyx in the adult human and mouse ocular surface
    Article Snippet: DNA oligonucleotides for lectin conjugation were 21 synthesized by Integrated DNA Technologies (Coralville, IA, USA).

    Staining:

    Article Title: Mutation of the GDP-Fucose Biosynthesis Gene gmds Increases Hair Cell Number and Neuromast Regenerative Capacity in Zebrafish.
    Article Snippet: Images were taken with a Nikon Brightfield Stereoscope (Nikon SMZ18, Tokyo, Japan). .. Aleuria aurantia Lectin (AAL, Vector Labs, Burlingame, CA, USA) stain was used to detect fucosylated proteins in the WT and gmds mutant embryos at 72 hpf. .. Live embryos were placed in centrifuge tubes containing 1 mL of 20 μg/mL AAL for 10 min and washed twice in PBST for 5 min, with larvae imaged on a Nikon SMZ18 microscope with a widepass green filter.

    Mutagenesis:

    Article Title: Mutation of the GDP-Fucose Biosynthesis Gene gmds Increases Hair Cell Number and Neuromast Regenerative Capacity in Zebrafish.
    Article Snippet: Images were taken with a Nikon Brightfield Stereoscope (Nikon SMZ18, Tokyo, Japan). .. Aleuria aurantia Lectin (AAL, Vector Labs, Burlingame, CA, USA) stain was used to detect fucosylated proteins in the WT and gmds mutant embryos at 72 hpf. .. Live embryos were placed in centrifuge tubes containing 1 mL of 20 μg/mL AAL for 10 min and washed twice in PBST for 5 min, with larvae imaged on a Nikon SMZ18 microscope with a widepass green filter.



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